Turning Flow Cytometry Upside Down and Inside Out: Cellular Function Revealed
Originally Aired May 1st, 2018
In the second webinar, you will learn how to apply basic concepts of flow cytometry to topics such as cell proliferation, dead cell exclusion, and intracellular staining techniques for transcription factors and phospho-proteins. In particular, you will learn the importance of:
- Titrating cell proliferation dyes and flow antibodies for optimal results
- How dead cell exclusion and staining temperatures of surface markers can influence interpretation of your data
- Different fixation and permeabolization buffers and their use in detecting transcription factors and secreted cytokines vs. phospho-proteins
An in-depth discussion of these techniques will be illustrated in a multi-parameter flow experiment examining human Memory T cell subsets.