Techniques and Best Practices for Cardiomyocyte Isolation
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Live Date: Thursday, November 1, 2018
Duration: 1 hour
In this educational webinar, Dr. Ronglih Liao and Dr. Davor Pavlovic discuss best practices for isolating healthy, calcium-tolerant adult cardiomyocytes for functional studies. They describe their techniques, the basic principles and theory behind those methodologies and any modifications that should be considered based on an experiment's species and reagents.
Specifically, Dr. Liao presents a proven, Langendorff-based method and discusses how to evaluate the success of an isolation based on calcium tolerance, morphology, and functional outputs.
Following, Dr. Pavlovic demonstrates an innovative Langendorff-free method for myocyte isolation, compares and contrasts it with the traditional procedure, and discusses the pros and cons of each.
This is an essential webinar for basic researchers utilizing isolated cardiac myocytes, those who have considered using them but have found the isolation procedure too daunting, and those who wish to improve their technique to produce more meaningful, reproducible data.
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