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Basic Principles of Clearing and Imaging Biological Tissues

Thursday, October 25, 2018
8AM PDT | 11AM EDT | 4PM BST | 5PM CEST

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Biological specimens are intrinsically three dimensional. Therefore, to fully understand their structure and function they are optimally imaged and viewed in this manner. However, because of the obscuring effects of light scatter, imaging deep into a tissue volume is problematic. Light microscopes were historically used to image individual cells or thinly sectioned tissue. Modern day confocal and multi-photon technologies extend the imageable volume to a few hundred micrometres but are still limited by light scatter. Efforts to eliminate light scatter by “clearing” tissue have been ongoing for over a century and have rapidly increased and expanded in recent years. Along with advances in imaging technology, tissue clearing can now allow for volume imaging over centimetres of depth.

This webcast will:
  • Introduce the physical basis for light scatter in tissue
  • Describe the mechanism underlying clearing techniques
  • Discuss several of the major advances in light microscopy for imaging cleared tissue

This webcast has been produced on behalf of the sponsor who retains sole responsibility for content. About this content
Presenter
Dr. Doug Richardson
Director of Imaging, Harvard Center for Biological Imaging
Harvard University
View Biography
Presenter
Dr. Jayshan Carpen
Moderator
Nature Research
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