FFPE Tissue and the HTG EdgeSeq Oncology Biomarker Panel: a Low-Input, Extraction-Free Gene Expression Pilot Study
The overwhelming majority of archival samples of cancer tissue are formalin-fixed, paraffin-embedded (FFPE). Many FFPE samples, especially pre-treatment biopsy specimens, are too small to reliably extract sufficient RNA for gene expression
analysis. In this webcast we look at a pilot study, which examined different amounts of tissue input from 3 colorectal and 2 breast tumors down to 3.1 mm2, representing a quarter of the recommended input.
During this webcast the speaker will address how:
- The HTG EdgeSeq Oncology Biomarker Panel provides a solid platform to obtain gene expression data from FFPE samples far too small for reliable RNA extraction.
- Reproducibility was robust, with technical replicates correlating >0.98, and small areas of the tumor correlating very well with each other, and reasonably well with NanoString and TaqMan data from extracted RNA.
- The lower limit for tissue input for this assay was not reached in this experiment, but most FFPE biopsy samples of interest will not be smaller than 3.125 mm2.
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