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FFPE Tissue and the HTG EdgeSeq Oncology Biomarker Panel: a Low-Input, Extraction-Free Gene Expression Pilot Study


The overwhelming majority of archival samples of cancer tissue are formalin-fixed, paraffin-embedded (FFPE).  Many FFPE samples, especially pre-treatment biopsy specimens, are too small to reliably extract sufficient RNA for gene expression
analysis. In this webcast we look at a pilot study, which examined different amounts of tissue input from 3 colorectal and 2 breast tumors down to 3.1 mm2, representing a quarter of the recommended input. 

During this webcast the speaker will address how:

  • The HTG EdgeSeq Oncology Biomarker Panel provides a solid platform to obtain gene expression data from FFPE samples far too small for reliable RNA extraction. 
  • Reproducibility was robust, with technical replicates correlating >0.98, and small areas of the tumor correlating very well with each other, and reasonably well with NanoString and TaqMan data from extracted RNA. 
  • The lower limit for tissue input for this assay was not reached in this experiment, but most FFPE biopsy samples of interest will not be smaller than 3.125 mm2.
You will also have the opportunity to ask questions of the speaker, live during the broadcast!
You will also have the opportunity to ask questions of the speakers, live during the broadcast!
 
Webcast sponsors retain sole responsibility for contentYou will also have the opportunity to ask questions of the speakers, live during the broadcast!You will also have the opportunity to ask questions of the speakers, live during the broadcast!

Webcast sponsors retain sole responsibility for content

Presenter
Dr. Katherine Pogue-Geile
Assistant Director of Molecular Profiling
National Surgical Adjuvant Breast and Bowel Project Foundation
View Biography
Presenter
Dr. Jayshan Carpen
Moderator
Nature Research
View Biography

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