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Magnetic 3D bioprinting, from organoids to fingerprinting cells in 3D using a 2D workflow 

Originally Aired: Wednesday, October 23, 2019
Speaker: Dr. Glauco R. Souza


This webcast has been produced by the Advertiser, Greiner Bio-One, who retains sole responsibility for content. 

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Overview

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The growing push for 3D cell culture models is limited by technical challenges in handling, processing, and scalability to high-throughput applications. To meet these challenges, we use our platform, magnetic 3D bioprinting, in which cells are individually magnetized and assembled with magnetic forces. In magnetizing cells, not only do we make routine cell culture and experiments feasible and scalable, but we also gain fine spatial control in the formation of spheroids and more complex structures. This presentation will focus on recent developments using this platform, particularly in cancer biology and immunology. Specifically, we will present methods and results for generating organoids for high-throughput screening (384- and 1,536-well), for phenotypic profiling of cell types within spheroids using real-time imaging and showing striking similarities between in vivo tissue and bioprinted cells. Overall, we use magnetic 3D bioprinting to create functionally and structurally representative spheroids for high-throughput screening.

Learning objectives:

  • General technique of magnetic 3D cell culture and magnetic 3D Bioprinting
  • Key advantages of 3D cell culture over other techniques
  • Broad applications of 3D cell culture
  • Applications in immunobiology
  • Comparison between magnetic 3D cell culture and in vivo results in collaboration with MD Anderson Cancer Center
  • HTS screening results in collaboration with Scripps Research Institute and Cold Spring Harbor
  • New approaches of visualizing 3D cell culture biology using real-time imaging
This webcast has been produced by Greiner Bio-One. The advertiser retains sole responsibility for content. About this content.

Presenters

Presenter
Dr. Glauco R. Souza
Director of Global Business Development and Innovation, 3D Cell Culture
Greiner Bio-One
View Biography