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Improving proteome coverage and peptide identification rates in short LC gradients

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This webcast has been produced for the sponsor, Thermo Fisher Scientific, who retains sole responsibility for content.

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Overview

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Keeping abreast of the ability to increase proteome coverage with advances in instrumentation and workflows is of great interest to proteomics investigators.

In this webcast, the speaker will present first impressions of the Thermo Scientific™ Orbitrap Exploris™ 480 mass spectrometer equipped with a Thermo Scientific™ FAIMS Pro™ interface for shotgun proteomics. The performance of the new instrument is evaluated in data-dependent acquisition (DDA) and data-independent acquisition (DIA) modes with fast scanning HCD methods in combination with front-end Field Asymmetric Ion Mobility Spectrometry (FAIMS).

This combination enables significantly increased proteome coverage when using single CVs and short LC gradients when connected to the Evosep One HPLC system. Moreover, the robustness of the new system is demonstrated by continuously evaluating peptide and protein identifications over thousands of LC-MS/MS analyses. Finally, raw sensitivity is evaluated analyzing dilution series of a HeLa digest by DIA, DDA and DDA with FAIMS.

You will learn:

  • What technology is required to have the deepest proteome coverage for shotgun proteomics
  • What methods are used to improve peptide identification rates in short LC gradients
  • What performance of the new instrument is expected for data-dependent acquisition (DDA) and data-independent acquisition (DIA*) modes
*DIA method is not available in the United States of America

 This webcast has been produced for ThermoFisher Scientific by Nature Research Custom Media. The sponsor retains sole responsibility for content. About this content.

Presenters

Presenter
Dr. Jesper V. Olsen
Professor, Novo Nordisk Foundation Center for Protein Research
University of Copenhagen
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Presenter
Moderator: Sarah Hiddleston
Journalist
Nature Middle East
View Biography