Place Cell Mapping and Stress Monitoring in
Head-Fixed Mice Navigating an Air-Lifted Homecage

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Overview

Live Date: Thursday, October 10, 2019

Time: 11:00 am Eastern Time (UTC -4)


In this webinar sponsored by Neurotar, scientists present data on 2-photon imaging of hippocampal place cells and on stress monitoring in head-fixed awake behaving mice.

Dr. Konrad Juczewski from the National Institutes of Health (NIH)/National Institute on Alcohol Abuse and Alcoholism (NIAAA) discusses the impact of head fixation on animals’ stress, locomotion and performance in classical behavioral paradigms.

Dr. Mary Ann Go from the Laboratory of Neural Coding and Neurodegenerative Disease at Imperial College London led by Prof. Simon Schultz presents her research using 2-photon microscopy aimed at place cell mapping in the hippocampus during exploration and navigation of a circular linear track or open-field arena.

Key discussion topics include:

  • Stress reduction in head-fixed rodents
  • Improving data reproducibility and translational value of the data acquired from head-fixed rodents
  • Effects of head fixation on blood corticosterone concentration, locomotion patterns and performance in stress-associated behavioral tests
  • Optimizing habituation protocol for head-fixed mice
  • Monitoring neural activity and mapping of place cells using 2-photon microscopy during navigation and exploration behavior
  • Automating the experiments using a closed-loop approach and behavior-triggered reward systems

Presenters

Presenter
Konrad Juczewski, PhD
Post-doctoral Fellow
National Institutes of Health/National Institute on Alcohol Abuse and Alcoholism (NIH/NIAAA)
Presenter
Ann Go, PhD
Postdoctoral Researcher
Neural Coding and Neurodegenerative Disease Laboratory, Imperial College London