Scalable and efficient AAV production process with new Fibro chromatography technology
July 30th 2020 08:00 PDT; 11:00 EDT; 16:00 BST; 17:00 CEST
Production methods for adenovirus-associated virus (AAV) vectors have not kept up with the brisk pace of gene therapy development. To manufacture safe and efficacious clinical-grade virus, scalable and cost-effective production processes are needed. Towards this end, we present an efficient process for AAV production and scale-up in suspension cell culture through to purified bulk product. The process was developed by evaluating and optimizing each process step.
A novel fiber technology, Fibro, addresses the downstream bottleneck at the capture step by overcoming the diffusional and flow limitations of purification using packed-bed chromatography. Also, a new analytical assay based on surface plasmon resonance was developed for AAV quantitation.
In this webinar our panel of experts will discuss:
- How to optimize each process step of AAV production and scale-up in suspension cell culture.
- How to evaluate and optimize cell culture conditions and plasmid transfection.
- Purification methods based on filtration and chromatography, developed for AAV production.
- The advantages of Fibro chromatography in downstream processing of AAV .