Sample Prep - An Optimized Workflow for Single-Cell Transcriptomics and Repertoire Profiling of Purified Lymphocytes from Clinical Samples

Previously Aired on May 13th, 2020

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Establishing clinically relevant single-cell (SC) transcriptomic workflows from cryopreserved tissue is essential to move this emerging immune monitoring technology from the bench to the bedside. However, uncontrollable factors in settings such as clinical trials can result in improper sample preparation during single-cell transcriptomic workflows, leading to loss of precious samples and compromised data. There is an urgent need to establish protocols specifically designed to overcome inevitable variations in sample quality in a clinical setting.

This webinar compares various sample preparation techniques for previously cryopreserved clinical samples and offers guidance to obtain high-quality single-cell data from human PBMCs in a variety of clinical settings. These include SC transcriptomic and repertoire analysis of a cryopreserved sample derived from a small amount of blood, with unknown or partially known preservation history.

Key Learnings:

• How to process cryopreserved, precious PBMC clinical samples for single-cell analysis

• Which cell counting method is ideal for single-cell workflows and how to improve viability of sample

• Why and how to enrich for T and B cells from frozen PBMCS prior to single-cell analysis


Richa Hanamsagar, PhD
Senior Scientist
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Victoria Reid
Director of Market Development
10x Genomics
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Mike Auerbach
American Pharmaceutical Review