Adventures with Imaging Flow Cytometry: Aging Cells, Giant Viruses and Golgi Fragmentation

November 7th, 2019

8am PT/11am ET


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In this webinar we will describe three recent applications for imaging flow cytometry (IFC), a technology that combines the information-rich imagery of microscopy with the quantification and high-throughput abilities of flow cytometry.

First we describe an automated method to quantify the senescence phenotype (a permanent state of cell cycle arrest), combining it with additional markers, improving the current qualitative methods. The second part describes using IFC to monitor changes in 
Acanthamoeba polyphaga cells infected with the giant DNA virus Mimivirus, including generation of viral factories, transport and fusion of replication centers within the cell, accumulation of viral progeny, and changes in cell morphology. The third part describes development of a method for using IFC to automatically quantify Golgi fragmentation, which plays an important role in numerous cellular processes. These examples demonstrate the diversity and versatility of IFC.


This webinar will:

  • Show the capabilities and unique advantages of the ImageStream
  • Review several examples of the versatility and diverse applications of the instrument
  • Explain how to quantify Golgi fragmentation, nuclear morphology, and cell senescence, and combine it with additional cellular processes.
Who should attend:
  • Cell biologists
  • Immunologists
  • Virologists
  • Oncologists
  • Flow cytometry core managers


Ziv Porat
Weizmann Institute of Science

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Tamlyn Oliver
Managing Editor
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