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Single-molecule proteomics

State of the art of single-molecule protein sequencing technologies

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Proteins are the workhorses in all living cells and, as such, form the basis of all living systems. In order to fully understand biological processes, it is critical to be able to identify and quantify the proteins in cells. This can be done by sequencing since each protein has a unique amino acid sequence. However, practical realisation of protein sequencing remains an enormous challenge.

Single-molecule techniques would revolutionize proteomics research, providing ultimate sensitivity for the detection of low abundance proteins, protein modifications and alternative splicing, paving a way to the realization of single-cell proteomics.

In this webcast, Dr. Chirlmin Joo of Kavli Institute of Nanoscience Delft will review recent novel approaches to single-molecule protein sequencing, together with the first experimental results from his group. He will discuss their advantages and drawbacks and share his perspective on the development of single-molecule protein sequencing techniques.

You will learn:

  • What types of single-molecule protein sequencing have been proposed
  • How proteins can be fingerprinted using single-molecule fluorescence
  • How peptides can be analyzed using nanopores  
This webcast has been produced on behalf of the sponsor who retains sole responsibility for content. About this content.


Chirlmin Joo
Associate Professor
Director Kavli Institute of Nanoscience Delft, Delft University of Technology
View Biography
Dr. Jayshan Carpen
Nature Research
View Biography