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Assaying genome-wide chromatin accessibility with ATAC-seq

Thursday, December 17th 2020
12pm EST, 5pm GMT

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Understanding the epigenetic regulation of gene expression in different cells and tissues is a key question in systems biology. Since it was first reported in 2013, Assay for Transposase Accessible Chromatin using sequencing (ATAC-seq) has been widely adopted as a sensitive, robust tool for genome-wide chromatin accessibility profiling. ATAC-seq has since been adapted for single-cell analysis to study cell type-specific chromatin accessibility in different tissues with heterogeneous cell populations.

This webcast will show participants how to use ATAC-seq and how it fits in with other chromatin accessibility profiling methods. First, William Greenleaf will discuss the development of ATAC-seq, its use, and adaptation to single cells. Bing Ren and Sebastian Preissl will then discuss single-cell ATAC-seq using combinatorial indexing, and application to brain, hearts and other tissues. These presentations will be followed by a live discussion with the speakers.

William Greenleaf
Associate Professor
Stanford University
View Biography
Bing Ren
Professor of Cellular and Molecular Medicine
University of California, San Diego
View Biography
Sebastian Preissl
Associate Director, single-cell genomics
Center for Epigenomics, UCSD
View Biography
Dominique Morneau
Chief Editor
Nature Reviews Methods Primers
Allison Doerr
Chief Editor
Nature Methods

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