Importance of Physiological Oxygen Levels for Improved Translation of Cell Physiology to Animal Models and Man
Physiological oxygen levels present in the vascular microenvironment in vivo range from 3 – 13 kPa, yet standard cell culture systems using traditional 5% CO2 incubators maintain cells in an atmospheric oxygen level of 18 - 21%.
Ongoing research, recently reviewed in our Physiological Reviews article (Keeley & Mann, 2019), has established that culture of endothelial cells under normoxic oxygen levels (5 kPa) exhibit altered regulation of Nrf2-regulated antioxidant genes, Ca2+ signaling and nitric oxide bioavailability compared to endothelial cells cultured under atmospheric oxygen levels.
It is important to highlight that the majority of published studies have employed conventional cell culture systems with an atmospheric oxygen level defined as “normoxia”, which does not correspond to the “normal” oxygen levels in vivo.
This webinar focuses on state-of-the-art techniques employed to accurately determine oxygen levels in living cells, as well as, the issues associated with reproducing physiological oxygen levels in vitro.
The webinar further aims to provide the framework for researchers to undertake cell culture under oxygen levels relevant to specific tissues and organs and thereby facilitate a paradigm shift, enabling translation of findings under physiological conditions in vitro to disease pathology and the design of novel therapeutics.