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Understanding cell functions in normal and tumorous tissue is a key factor in advancing research of potential treatment strategies, and understanding why some treatments might fail. Single-cell analysis is crucial in biomedical research to unravel which cellular and molecular pathways are altered in complex diseases such as cancer.
In this webcast, the speaker will show how improved fluorescence microscopy imaging techniques help researchers acquire more relevant data for the analysis of organelle membrane cell dynamics.
She will present techniques that helped to reveal that chemoresistant ovarian cancer cells display higher levels of cholesterol (labelled with filipin dye) on the plasma membrane compared to their sensitive counterpart, and that intracellular filipin-dots colocalizing with lysosomes have significant increases in chemoresistant cells, indicating that these cells take up much more cholesterol. This is consistent with higher expression of LDL receptor.