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Nanion Technologies - Webinar


Date: 19. May 2020

Time: 4:00 PM CET / 10:00 AM EDT 

Nathan Thomas
"Unlocking the (Reversal) Potential of SSM Electrophysiology: Transporter Stoichiometry with the SURFE²R N1” 

Abstract: Mechanistic studies of ion-coupled transporters can be facilitated if the stoichiometry of the transport reaction is known, but current methods for measuring transport stoichiometry are both material and labor intensive. We developed an SSM-based electrophysiology reversal potential assay using the SURFE²R N1. This assay allows for fast, accurate determination of transport stoichiometry without the need for radioactivity or fluorescent probes while using only picomols of protein. We report our results with GdX, aguanidinium exchanger with a known 2:1 proton:guanidinium antiport stoichiometry.“

Camillo Perez:
"Characterization of a choline uniporter by SSM-based electrophysiology"

Abstract: My group studies multiple transport proteins involved in the biosynthesis of teichoic acids in Gram-positive bacteria. We aim to understand the mechanism of these proteins and to describe their importance for bacterial growth, adaptation, and survival, as they are considered valuable drug targets. Some Gram-positive bacteria display teichoic acids with a phosphorylcholine modification that serves as an anchor for choline-binding proteins (CBP). CBPs play fundamental roles in interactions with biological surfaces and in response to external threats. The growth of these bacteria frequently depends onexogenous choline, which is metabolized to modify teichoic acids. Transporters located at their plasma membrane harvest large amounts of choline from the external medium. We are using SSM-based electrophysiology to characterize the function of some of these transporters. SSM-based electrophysiology allows us to perform high-throughput screening of multiple chemical species, providing the biochemical basis for the production of novel inhibitors of Gram-positive bacteria choline transporters.


Maria Barthmes
Product Manager
Nanion Technologies
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Nathan Thomas
PhD Student
University of Wisconsin-Madison
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Camilo Perez
Prof. Dr.
Biozentrum - University of Basel
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