Basic Principles of Clearing and Imaging Biological Tissues
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Biological specimens are intrinsically three dimensional. Therefore, to fully understand their structure and function they are optimally imaged and viewed in this manner. However, because of the obscuring effects of light scatter, imaging deep into a tissue volume is problematic. Light microscopes were historically used to image individual cells or thinly sectioned tissue. Modern day confocal and multi-photon technologies extend the imageable volume to a few hundred micrometres but are still limited by light scatter. Efforts to eliminate light scatter by “clearing” tissue have been ongoing for over a century and have rapidly increased and expanded in recent years. Along with advances in imaging technology, tissue clearing can now allow for volume imaging over centimetres of depth.
This webcast will:
- Introduce the physical basis for light scatter in tissue
- Describe the mechanism underlying clearing techniques
- Discuss several of the major advances in light microscopy for imaging cleared tissue
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