Leveraging the power of high-parameter cell sorting and single-cell multiomics to profile intratumoral immune cells in a model of B cell lymphoma

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Overview

The pathogenesis of B cell malignancies is a dynamic process involving reciprocal interactions between malignant cells and surrounding components in the tumor microenvironment. In the case of certain B cell lymphomas, neoplastic cells migrate to lymphoid sites and prompt the infiltration of non-malignant immune cells. The infiltration of cytotoxic lymphocytes correlates with tumor cell killing and good prognosis. However, most immune cell types in the tumor niche often become unresponsive or end up promoting tumor growth.

This webinar will describe a detailed workflow for successful sorting of six immune cell populations from a tumor niche in a mouse model of B cell lymphoma and it will uncover strategies for increasing sample throughput. It will also demonstrate how to perform a detailed examination of critical cell populations in tumor responses using simultaneous analysis of proteins and mRNA transcripts at the single-cell level, especially for rare subsets.

Key topics discussed in the webinar will include:

• Steps for cell preparation before 6-way sorting to reduce cell stress and cell loss

• Strategies to increase sample throughput by enabling sample multiplexing and concurrent multiparametric analyses of surface proteins and mRNA transcripts

• Exploiting Uniform Manifold Approximation and Projection (UMAP) and other easy-to-use utility tools for demultiplexing samples and deep single-cell analysis

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Presenters

Presenter
Gisele Baracho, PhD
Staff Scientist, Applications Development

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Presenter
Alejandro Montenegro-Montero, PhD
Current Protocols
Wiley
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