Met-Flow: A Strategy for Single-Cell Metabolic Analysis

October 21, 2020 at 1:00 pm SGT

A complex interaction of anabolic and catabolic metabolism underpins the ability of leukocytes to mount an immune response. Their capacity to respond to changing environments by metabolic reprogramming is crucial to effector function. However, current methods lack the ability to interrogate this network of metabolic pathways at a single-cell level within a heterogeneous population. Using Met-Flow, a flow cytometry-based method, the metabolic state of immune cells can be captured by targeting key proteins and rate-limiting enzymes across multiple pathways.

New technologies including antibody panels, flow cytometers and advanced data-analysis tools have been developed to simultaneously measure divergent metabolic profiles and dynamic remodeling in human peripheral blood mononuclear cells. This webinar will provide insight into the complex metabolic state of any cell as it relates to phenotype and function, leading to a greater understanding of the role of metabolic heterogeneity in immune responses.

Key topics covered in this webinar include:

• How staining and analysis of metabolic and cell surface proteins can be made simultaneously at a single-cell level

• How a novel high-parameter flow cytometry method utilizing antibodies against metabolic proteins that are critical and rate-limiting in their representative pathways can shed light into the metabolic state of any given cell type

• How various advanced data-analysis tools can be utilized to derive unique correlations between immune subpopulations and specific metabolic pathways

Who should attend:

• Core facility directors and research investigators engaged in immune monitoring studies

• Researchers in immuno-oncology

• Anyone interested in learning more about applying flow cytometry and advanced data-analysis tools to understand the role of metabolic heterogeneity in immune response

For Research Use Only. Not for use in diagnostic and therapeutic procedures.

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John Connolly, PhD
Research Director, Institute of Molecular and Cellular Biology
Chief Scientific Officer, Parker Institute for Cancer Immunotherapy
Director, Program in Translational Immunology
Adjunct Associate Professor, Baylor University

View Biography

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