High-Throughput Multiplexed Screening of TCR Signaling Inhibitors
Originally Aired October 30th, 2018
Now available on-demand
T cells play a critical role in the adaptive immune response. In naive T cells, binding of the T-cell receptor (TCR) to peptides complexed with major histocompatibility complex triggers an intricate signaling mechanism leading to T-cell activation, proliferation, and production of cytokines. Modulating the TCR signaling pathway using biologics, small molecules, or genetic engineering is highly relevant to many therapeutic areas, including cancer immunotherapy, adoptive cell therapy, vaccine development, and autoimmune disease. The development of these drugs and therapies require the routine use of assays to profile T-cell function and health. Join us for this GEN webinar where we will discuss the importance of routine monitoring of T-cell function in drug discovery. Moreover, we will highlight the need for rapid immune-function monitoring and how this can be addressed by simultaneously measuring cell phenotype, T-cell activation markers, cell proliferation, cell viability, and quantifying secreted cytokines in a miniaturized assay format.
- High-throughput screening of a library of kinase inhibitors for T-cell activation inhibition using primary human PBMCs
- Evaluating CD69, CD25, and HLA-DR activation markers, cell proliferation and IFN-γ and TNF in a single multiplexed assay
- Deriving actionable insights from powerful data analysis tools, such as heat maps and IC50 curves, and combining assay outcomes to help quickly locate hits using multiplexed criteria
A live Q&A session will follow the presentation, offering you a chance to pose questions to our expert panelist.