Fluorescence activated cell sorting (FACS) and mass cytometry are widely applied technologies for exploratory immune profiling and biomarker discovery. Peripheral blood mononuclear cell (PBMC) samples are often studied by measuring T-cell and antigen presenting cell (APC) focused antibody panels developed for these technologies.
As single-cell RNA sequencing (scRNA-Seq) technologies are being increasingly implemented in various areas of disease research, novel applications have emerged that allow for the simultaneous profiling of different -omics read-outs in the same single cell. One such multimodal read-out, termed multiomic cytometry, is the combination of measuring the transcriptome with protein surface marker information by using antibodies that are conjugated to a DNA barcode.
In this webinar, we will present a detailed overview and analysis of data generated from healthy volunteer PBMC samples utilizing a 14-antibody FACS panel, a 62-antibody mass cytometry panel, and overlapping multiomic cytometry panel to provide a better understanding of the concordance among these different technologies.
In this webinar you’ll learn:
• The features and benefits of multiple cytometry techniques: multiomic cytometry, flow cytometry, and mass cytometry
• How you can leverage single-cell partitioning, coupled with DNA barcoding and next-generation sequencing (NGS), to achieve ultra-high parameter phenotyping of individual immune cells
• How to improve biomarker discovery by deeply characterizing cell types through the simultaneous analysis of cell-surface epitopes and intracellular mRNA-based gene expression parameters in up to tens-of-thousands, or more, individual cells
Who should attend:
• Researchers interested in gaining a better understanding of cellular phenotyping and characterization
• Researchers who would like to expand the number of measurable parameters in single cells
• Those who would like to learn about how to better leverage single-cell multiomic sequencing to benefit their cellular research