Getting a Grasp on Multimodal Single Cell Omics Data:
In-Depth Comparison Between Multiomic Cytometry and Traditional Cytometric Methods

June 22nd, 2020 • 9 am PDT, 12 pm EDT 

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Overview

Fluorescence activated cell sorting (FACS) and mass cytometry are widely applied technologies for exploratory immune profiling and biomarker discovery. Peripheral blood mononuclear cell (PBMC) samples are often studied by measuring T-cell and antigen presenting cell (APC) focused antibody panels developed for these technologies.

As single-cell RNA sequencing (scRNA-Seq) technologies are being increasingly implemented in various areas of disease research, novel applications have emerged that allow for the simultaneous profiling of different -omics read-outs in the same single cell. One such multimodal read-out, termed multiomic cytometry, is the combination of measuring the transcriptome with protein surface marker information by using antibodies that are conjugated to a DNA barcode.

In this webinar, we will present a detailed overview and analysis of data generated from healthy volunteer PBMC samples utilizing a 14-antibody FACS panel, a 62-antibody mass cytometry panel, and overlapping multiomic cytometry panel to provide a better understanding of the concordance among these different technologies.

In this webinar you’ll learn:

• The features and benefits of multiple cytometry techniques: multiomic cytometry, flow cytometry, and mass cytometry

• How you can leverage single-cell partitioning, coupled with DNA barcoding and next-generation sequencing (NGS), to achieve ultra-high parameter phenotyping of individual immune cells

• How to improve biomarker discovery by deeply characterizing cell types through the simultaneous analysis of cell-surface epitopes and intracellular mRNA-based gene expression parameters in up to tens-of-thousands, or more, individual cells

Learn more at 10xgenomics.com & BioLegend.com.

Who should attend:

• Researchers interested in gaining a better understanding of cellular phenotyping and characterization

• Researchers who would like to expand the number of measurable parameters in single cells

• Those who would like to learn about how to better leverage single-cell multiomic sequencing to benefit their cellular research

Presenters

Presenter
Hans Wils, PhD
(Presenter)
Senior Scientist, Single Cell Sequencing Technologies
Janssen Pharmaceutical Companies of Johnson & Johnson
View Biography
Presenter
Tamlyn Oliver
(Moderator)
Managing Editor
Biocompare
View Biography