Fluorescence activated cell sorting (FACS) and mass cytometry are widely applied technologies for exploratory immune profiling and biomarker discovery. Peripheral blood mononuclear cell (PBMC) samples are often studied by measuring T-cell and antigen presenting cell (APC) focused antibody panels developed for these technologies.
As single cell RNA sequencing (scRNA-seq) technologies are being increasingly implemented in various areas of disease research, novel applications have emerged that allow for the simultaneous profiling of different omics readouts in the same single cell. One such multimodal readout, termed multiomic cytometry, is the combination of measuring the transcriptome with protein surface marker information by using antibodies that are conjugated to a DNA barcode.
In this webinar, we will present a detailed overview and analysis of data generated from healthy volunteer PBMC samples utilizing a 14-antibody FACS panel, a 62-antibody mass cytometry panel, and an overlapping multiomic cytometry panel to provide a better understanding of the concordance among these different technologies.
You will learn about
• The features and benefits of multiple cytometry techniques: multiomic cytometry, flow cytometry, and mass cytometry
• How you can leverage single cell partitioning, coupled with DNA barcoding and next-generation sequencing (NGS), to achieve ultra-high parameter phenotyping of individual immune cells
• How to improve biomarker discovery by deeply characterizing cell types through the simultaneous analysis of cell-surface epitopes and intracellular mRNA-based gene expression parameters in up to tens of thousands, or more, individual cells
Who should attend:
• Researchers interested in gaining a better understanding of cellular phenotyping and characterization
• Researchers who would like to expand the number of measurable parameters in single cells
• Those who would like to learn how to better leverage single cell multiomic sequencing to benefit their cellular research